ME MY LITTLE HELPER

Brand	Owner	Address	Description
"ME & MY LITTLE HELPER"	Midwest Quality Gloves, Inc.	835 Industrial Road Chillicothe MO 64601	ME AND MY LITTLE HELPER;GARDEN AND YARD WORK GLOVES, AND HOUSEHOLD GLOVES FOR GENERAL USE;
	Where the owner name is not linked, that owner no longer owns the brand

Technical Examples

The present invention provides a helper cell for expressing an infectious, replication defective, alphavirus particle in an alphavirus-permissive cell. The helper cell includes (a) a first helper RNA encoding (i) at least one alphavirus structural protein, and (ii) not encoding at least one alphavirus structural protein; and (b) a second helper RNA separate from the first helper RNA, the second helper RNA (i) not encoding the alphavirus structural protein encoded by the first helper RNA, and (ii) encoding the at least alphavirus one structural protein not encoded by the first helper RNA, such that all of the alphavirus structural proteins assemble together into alphavirus particles in the cell. Preferably, the helper cell also includes a replicon RNA encoding an alphavirus packaging sequence and an inserted heterogeneous RNA.